taken from "Desmontar el SIDA" n.2 - COBRA


D. You state that the HIV virus does not exist. If it is so, how is it possible that lots of scientists in the world are carrying out experiments with the HIV?
R. Only because they agree about one concept. Some of them believe that when in certain cellular cultures an inverse trascriptasis activity is present, it indicates the presence of a virus. When some other scientists find DNA fragments, they believe that they come from a virus. Others, if they locate proteins like the P24 or the P41, believe that they belong to a virus, or they point out that they have isolated a virus.
To isolate a virus means to separate it from any other thing. We are talking of a procedure that has been carried out with all the known viruses. It is easy to carry out since a virus, unlike cells, has always the same size and the same shape.
One part of the isolation process of a virus is centrifugation. If a solution where viruses are present is being centrifuged, we will have as a result a dark sediment with a ring shape, which is formed by viruses. Once a solution with viruses has been centrifuged, a sample is taken, coloured and photographed with an electronic microscope. Even the electroforesis is part of the procedure of isolating a virus. This test allows to isolate different proteins of the virus according to their size. In order to do so, at first a sample of viric particles is taken and broken up.
D. How can a virus be broken?
R. With detergent, which dissolves the outer covering of the virus, more technically the lipidic layer. A detergent called SDS with a negative charge is used.
D. What has to be done next?
R. A negative electric charge is transmitted to these "pieces" and they are displayed in a gel above a plastic film, which is in turn exposed to an electric field; positive charge to the lower edge and negative to the upper one.
Due to the electric field, the proteins contained in the broken virus with a negative charge move from the bottom to the top, but with a different speed. This is due to the difference of weight and seize. The proteins of the same size gather in layers; the biggest ones, which move with more difficulty, gather next to the positive pole and the smaller ones to the negative pole.
At this point the gel is coloured and dried. As a result, one gets a plastic film with different proteins of the same type. Curiously a photograph of the proteins has never been taken , neither of the HIV nor of any retrovirus.
Viric particles have never been shown in a test-tube, after centrifugation.
The only thing they have done is to determine inverse transcriptasis activity. They speak about proteins but they do not show the gel.
At this point of the test, the gel is transferred into nitrocellulose or into a nylon membrane. A sample of blood is added and if it contains antibodies of any of these proteins, these antibodies combine with the respective protein. This combination can be seen through a method of colouring or through radioactivity impressed, printed in a film (similar to a small x-ray). Marks or spots show where the antibodies join the proteins. If these can be seen in the film, they say that the result of the test is positive.
Actually this does not mean anything so specific since in the first place they hide the fact that what turns up are not proteins but antibodies combined to proteins instead.
To be positive to ELISA or WESTERN BLOT tests does not make sense, since it has not been shown that the proteins are those of the HIV and besides the HIV has never been isolated. The only thing that can be supposed is that these proteins come from leukocytes.
At this point a line of "special" cells becomes necessary (that have grown out of the body) as well as leukocytes from the body. They are mixed and stimulated with oxidizer.
If there is inverse transcriptasis as a result of this combination, they state that the virus is present in the leukocytes of the body.
Certainly, what is done is to stimulate cells to such a point of stress, a situation that cannot take place in the human body. This provokes the production of proteins that would not be produced in normal conditions. These are the proteins that are separated and the test's kits are made with them. If there have been contacts with this type of proteins of leukocytes (through transfusions, transplantation, factor VII) or if sperm has been received through the rectum (which contains leukocytes), the test can turn positive.
D. If the HIV has never been isolated what do the photographs into circulation show about the so-called virus?
R. These photographs do not demonstrate anything. What can be seen in these photographs are ultra-thin sections of these proteins that are supposed to be those of the HIV. However, in the same photographs other particles turn up, that is why it cannot be stated that they come from an isolated virus.
In order to make these photographs, a different procedure from the one described before must be carried out (that is, what is considered by everyone the scientific proof of the existence of a virus).
Resin to fix the proteins is added to the fluid containing the culture of cells. It is dried with alcohol and it is coloured, the resin is polymerized and finally cut in ultra-thin layers. These layers are photographed with the electronic microscope.
However, in this case we are not talking about a virus, but about cellular particles that the cell itself produces, which grow inside the cell itself and that leave and turn to other cells to enter them afterwards. They are not steady particles, they are only useful for intracellular transport, they are not produced outside the body. They use the glicoproteins contained in its surface to combine with other cells. These processes are called exocitosis and endocitosis.
D. How is the inverse transcriptasis demonstrated?
R. It is an interesting subject since this is not dealed correctly either. The inverse transcriptasis transcribes RNA into DNA. The procedure is the following: they add letters (nucleotids) of radioactive DNA to the RNA. If there is inverse transcriptasis new DNA will be formed, which, in turn, will obviously be radioactive. This new DNA is passed through a filter that does not allow the flow of radioactive letters. If radioactivity is found in the filter, they explain that there has been inverse transcriptasis, therefore they finish stating that the virus is present.
However, this has two problems:
1. the RNA sample is the same used for polimers of DNA in "culture" (in vitro), therefore it is not certain that what has been found is specific inverse transcriptasis;
2. there is always inverse transcriptasis in other cellular reactions that are not taken into consideration.
In fact, the way the experiments are carried out is the following: after drawing the "co-culture" the proteins are separated. In order to do so, the fluid is taken and centrifuged to separate the cells, so as to have only virus remaining in a certain part of the test-tube. A fluid of remarkable density is added to this fluid containing virus, it is mixed and centrifuged. By means of centrifugation the particles concentrate and sediment in different layers, according to their density.
In fact, at the same time, a check experiment with non-infected material should be carried out , in order to show that what has been isolated are not cellular particles. In these check experiments, carried out in the same way and following the same procedure, nothing isolated should turn up.
Afterwards, in order to demonstrate inverse transcriptasis (taking into account that we are taking as a dogma that if there is inverse transcriptasis there must be a retrovirus), samples of the different parts of the sediment in the test-tube are taken and tests are carried out to determine the presence of inverse transcriptasis.
RNA and nucleotids turned radioactive are added to every layer of particles. If there is inverse transcriptasis DNA will be produced. The new DNA, in turn, is radioactive. It is filtered and washed, in order that what remains in the filter is radioactive DNA, then the radioactivity is measured. As a result of the test we have a design where the peak of the curve indicates the more radioactive sample. These are the particles that are considered those of the virus and that are used in the above mentioned tests.
Stating that the presence of inverse transcriptasis implies the existence of the virus does not make sense, considering that since 1981 they have known that the inverse transcriptasis could be found in other places, and we already know that there are other enzymes that are able to produce DNA out of RNA.
D. What is and how is the PCR used?
R. It means polimerase chain reaction; it is a procedure that is useful to multiply DNA. Usually in order to duplicate DNA an experiment in vitro is carried out putting DNA, radioactive nucleotic polymer and initial molecules. Finally, as at the beginning, the presence of DNA is determined by measuring the radioactivity in the filter. The problem is that all these tests can be carried out only once.
In order to produce DNA a fragment of simple DNA is taken. As one knows, the
DNA is formed by a double helix, one side positive and the other negative. The four types of nucleotids combine among themselves always in the same way. In order to duplicate DNA the helix has to be split for the single fragments to remain (not the double ones). Besides, an initial molecule is necessary. The polimerase combines with the initial molecule with the simple fragment of DNA and new DNA starts to turn up (in a double helix). If we have used radioactive nucleotids, the DNA in turn will be radioactive. Once the DNA is formed the reaction stops.
If the initial amount of DNA was small, the presence of DNA could be difficult to determine due to the small amount. In order to multiply and therefore overcome this problem the PCR is used. The PCR uses the property of certain polimers of standing high temperatures. If the DNA is warmed after the production, the DNA with a double helix become split in single fragments. A normal polimerasis would be destroyed after being warmed, but the polimerasis of certain bacteria of the bottom of the sea are resistant to high temperatures (up to 90°). In this way, after the polimerasis has produced new DNA, it is warmed for the double helix to separate and it is cooled down so as to the polimerasis will be ready to work again.
Once the mixture is cold, the polimerasis forms again DNA and afterwards it is warmed again for the helix to break again. Each warming-cooling operation doubles the amount of DNA. Therefore, by repeating the operation different times a lot of molecules are produced, which can be shown with electroforesis.
If one wants to know if someone has the HIV, an initial molecule of twenty letters has to be produced which could only combine itself with an HIV gene. If DNA is produced it means that the virus is there.
The problem is that with this test only 500 letters can be found (the HIV is supposed to have 10000). However, it is believed that 500 is enough.
Besides, it is well known that these small fragments are very similar to the ones that are found in the human being.
In fact, the "human genoma" project reached the conclusion that there is about 90% of repetitive sequences in the human being. It has also been demonstrated that some of these sequences are very similar to those ascribed to retroviruses. By means of the PCR only the known sequences can be multiplied since the initial molecule must be similar to the molecule of the DNA fragment.
Only a known sequence can be multiplied, since a molecule of 20 letters has to be synthesized which is the same as the one that has to be amplified. This molecule has to produce the initial combination.
Since HIV virus has never been isolated, therefore it is not known, the result of the PCR is at least doubtful. Therefore, how can it be stated that the DNA that is used comes from the HIV?
It cannot be stated that a positive PCR result indicates the presence of a virus. Consequently, lately the sequences of the HIV are not longer being published.
The PCR can have a positive or a negative result according to the way it is carried out. There are not enough check tests. What do they do, for instance, to get a positive result? In order to determine the amount of virus, they take cells from the body and from stress cultures and they isolate their RNA. It is very important to understand that these conditions do not exist in any way in a human being. When the cells are in a state of stress in a highly oxydized environment, they produce particles that would not produce in normal conditions. If a cell dies, among the big amount of substances it produces, there is a lot of RNA.
If cells coming from healthy people are taken, RNA can hardly be found. On the contrary, if cells coming from sick people are taken, for instance with flu or in a state of stress, RNA is found and therefore an ensuing test can have a positive result. In order to make this test, the RNA is transcribed into DNA, which is used to carry out the PCR. Afterwards, the initial molecule is designed and the reaction will probably take place.
In order to have a negative result, two things can be done:
1. the DNA of the person is taken straightforward and the initial molecule is placed: there is no reaction! Why? Since the DNA has some fragments with genetic information and others without it, therefore if the initial molecule does not react, it will not work;
2. RNA from non-stressed cells is taken, and therefore there will not be any sequence (stressed sequences are produced only by stressed cells).
D. What does the "template switching" consists of?
R. It is a characteristic of the inverse transcriptasis, which combines separate fragments of DNA forming long chains of DNA. The usual mistake is the belief that this DNA belongs to the HIV.
D. What can be said about the T4/T8?
R. The immunologists have not obtained anything trying to find useful instruments to diagnose the B cells.
There are thousands of articles that demonstrate this.
Today monoclonal antibodies are used to identify the different T cells. The monoclonal antibodies only combine to a certain protein. They were produced for the first time in 1975. They obtained twenty four monoclonal antibodies which stuck to the surface of the T cells. In this way they started to call the cells CD, CD2, CD3, CD4...CD8, and so on.
CD means "Cluster of Differentiation", that is, group of differentiation. Nothing else.
The experiments started with mice that had received radiation, injecting them human T cells in order to study the human immunity system. They came to the conclusion that some cells were "killers", some "helpers" and other "suppressors". However, this is not correct, it is only an interpretation.
Actually, looking closely at the T cells, it is not easy to distinguish them.
They carried out experiments in order to examine the behaviour of these cells and to be able to see what kind of receptors they had in their surface. But actually there are different types of proteins in the surface of the cells. Sometimes more, sometimes less.
This fact could explain why the people that use drugs (which are oxidizers) have a lower amount of T4 in the surface of the cells. It is important to understand that if there is stress, a great deal of the leukocytes of the blood migrate towards the spinal cord (the maximun amount of leukocytes present in the blood does not exceed 5% of the total, the remaining 95% is in the tissues) also because the role of the leukocytes is to carry antigenes to the spinal cord. It has been shown that the corticoids produced during stress migrate towards the tissues and cannot be found in the blood. This fact makes it clear why stressed people have a low amount of T4 (the people considered AID's patients believe to be time bombs). It is well known that athletes have very low levels of T4 and in long-distance runners they can hardly be found. Besides, the people that carry out hard manual work have very low levels of T4.
From the point of view of evolution, if it is necessary to hunt or to escape from a danger, it does not make sense that the energy of the body is used to produce leukocytes. If there is a lot of stress, as in the case of a sprinter, once the effort is carried out, T cells are not found.
This shows the mistake of the AIDS' definition of 1992 in the United States. It points out that also in the case of a negative test, if the amount of T4 is below 200 the diagnosis is AIDS.
Regarding the relationship between T4 and T8, Dr. Eleni Papadopulos-Eleopulos makes it clear in detail.
In case of stress there are more proteins T4 than T8 in the surface of the cells.
Fauci, a well known official exponent of the AIDS studies, had shown that during stress the leukocytes migrate to the tissues; this is very significant, taking into account the role of this gentleman in the subject.
D. Are there subjective factors that reinforce the HIV-AIDS idea?
R. Yes, all viruses and bacteria have a negative image.
If viruses are present in the food we eat, we consider it wrong. If we undergo an operation and we get an infection, we consider it wrong as well. In ordinary infections, viruses and bacteria have a positive role (for instance, they help to disperse degenerate tissues). This has not been studied enough.
It is a remarkable improvement to change the way of thinking.
D. What can you say about the flu virus?
R. This virus exists. It was isolated. It seems to help disperse damaged tissues.
D. Therefore, does it help?
R. Yes, everyone knows that with or without a medical treatment the flu is defeated. If the body is tired it needs to recover, this is not a problem. It is always better to avoid antibiotics in general.
D. How do you imagine the future of AIDS?
R. After the war to cancer launched by Nixon in the USA in 1961, which failed in 1970, the cancer virologists became AIDS virologists. We can say that the AIDS is the small sister of the cancer research. I hope it does not grow much. I hope there will not be much future for AIDS.
We must put pressure on politicians, accuse them of not preventing and of not stopping the death of their own citizens. We have to accuse them of taking part in the killing of "third world" people by supporting AIDS programmes.